Figure 2.

Representative gel images of RT-PCR of RNA isolated by laser capture microdissection for GAT subtypes 1–4. (A) Human airway epithelium from tracheal rings. (B) Human ASM from tracheal rings. Encoding mRNA for GAT subtypes 2 and 4 were detected in RNA isolated from pure human airway epithelium and smooth muscle cells, whereas mRNA for GAT1 and GAT3 were not. Images are representative of experiments performed on RNA isolated from two to three different native guinea pig or human tissues. HASM, human ASM from tracheal ring; HBr, human brain (positive control); HEpi, human airway epithelium from tracheal ring; MW, 100 base pair ladder. Water denotes the negative control (no cDNA input).