Skip to main content
. 2010 Nov 5;45(2):332–339. doi: 10.1165/rcmb.2010-0177OC

Figure 3.

Figure 3.

Representative gel images of RT-PCR of glutamic acid decarboxylase (GAD) isoforms GAD65 and GAD67 from RNA from freshly dissected native human and guinea pig ASM and epithelial tissue, and cultured human ASM and epithelial cells. mRNA for GAD65 is present in guinea pig ASM, whereas mRNA for GAD67 is present in native and cultured human ASM and epithelium. Images are representative of experiments performed on RNA isolated from two to three different native guinea pig or human tissues or two to three cultured cell flasks. 1, 100 base pair ladder; 2, water, which denotes the negative control (no cDNA input); 3, native human ASM; 4, native human airway epithelium; 5, cultured human ASM cells; 6, cultured human epithelial cells; 7, human brain; 8, native guinea pig ASM tissue; 9, native guinea pig airway epithelial tissue; and 10, guinea pig brain. Separate gel images are denoted by a solid demarcating line.