Figure 5.

After-exposure antioxidants decrease BAL albumin and IgM in mice exposed to Cl₂. Mice were exposed to 600 ppm of Cl₂ for 45 minutes and returned to room air and treated with either ascorbate and deferoxamine (Cl₂ Asc/Def) or vehicle (Cl₂ Vehicle), as described in Materials and Methods. Surviving mice were killed 72 hours after Cl₂ exposure and used for these measurements. A third group of mice was exposed to air (Air Control) and did not receive antioxidants. Equal volumes (40 μl) of BAL were separated by 10% SDS-PAGE and transferred polyvinylidene difluoride (PVDF) membranes and blotted for (A) albumin and (B) IgM. Bands were digitized and mean values are shown in C and D. Values of densitometry units are expressed as means (±SE). Shown are results of a typical experiment.