Figure 6.
After-exposure antioxidants decrease number of cells in BAL in mice exposed to Cl2. Mice were exposed to 600 ppm of Cl2 for 45 minutes, returned to room air, and treated with either ascorbate and deferoxamine (Cl2 Asc/Def) or vehicle (Cl2 Vehicle), as described in Materials and Methods. Surviving mice were killed 72 hours after Cl2 exposure and used for these measurements. A third group of mice was not exposed to Cl2, and did not receive any treatment serving as baseline. After being killed, the lungs of mice were lavaged and BAL samples were spun at 300 × g for 10 minutes at 4°C to pellet cells. (A) Total number of cells in BAL; (B) the indicated types of inflammatory and epithelial cells. Values are means (±SEM); number of animals in each group: (A) Air Control, 18; Cl2 Asc/Def, 9; Cl2 Vehicle, 4; (B) Air Control, 7; Cl2 Asc/Def, 9; Cl2 Vehicle, 4. Statistical analysis was performed after square root transformation of the data to meet assumption of normal distribution, followed by one-way ANOVA and Tukey multiple comparisons post test (A), and two-way ANOVA and Bonferroni post test. Values are expressed as means (±SE). *P < 0.001 compared with air control; #P < 0.001 compared with Cl2 vehicle.