Figure 8.

Ascorbate and deferoxamine treatment decreased BAL lipid peroxidation in Cl₂-exposed mice. Mice were exposed to 600 ppm of Cl₂ for 45 minutes, returned to room air, and treated with either ascorbate and deferoxamine (Cl₂ Asc/Def) or vehicle (Cl₂ Vehicle), as described in Materials and Methods. Surviving mice were killed 72 hours after Cl₂ exposure and were used for these measurements. A third group of mice was exposed to air and did not receive any treatment. Equal amounts of proteins (5 μg) from cell-free BAL were separated by 10% SDS-PAGE and transferred to PVDF membranes. Lipid peroxidation was detected using a primary rabbit anti-malondialdehyde (MDA) antibody. Values of densitometry units are expressed as means (±SE). ^#P < 0.05 versus Cl₂ saline. Statistical analysis was performed after square root transformation of the data to meet assumption of normal distribution, followed by one-way ANOVA and Tukey multiple comparisons post test. Number of animals in each group was: Air Control, 2; Cl₂ Asc/Def, 4; Cl₂ Vehicle, 4.