Figure 3.

Effects of Cl₂ on aortic endothelial nitric oxide synthase (eNOS) expression eNOS mRNA (A) or protein (B and C) were determined in aorta isolated from rats 24 hours after exposure to air or Cl₂ (400 ppm, 30 min). eNOS protein levels were determined by immunoflouresence (B) or by Western blotting (C; inset shows representative Western blots). Data show mean ± SEM (n = 3–4). **P < 0.05, *P < 0.03, and ^#P < 0.001 relative to control. (D) Plasma nitrite concentrations in 24 hours after air or Cl₂ exposure. Data show mean ± SEM (n = 5–7). ^#P < 0.05 relative to control.