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. 2010 Dec 3;45(3):453–458. doi: 10.1165/rcmb.2008-0393OC

Figure 4.

Figure 4.

HA-SRF binds KPNA1-myc and KPNA2-myc in vivo. (A) Immunoprecipitation of nuclear extract with anti-HA beads (pulls down SRF) and Western blot with anti-myc antibody (karyopherin) demonstrates in vivo interaction of SRF with KPNA1 but not with KPNA2–4 in transfected 293 cells. Equal amounts of nuclear protein were loaded in each input (I) lane. (B) Immunoprecipitation of cytoplasmic proteins with anti-HA beads (pulls down SRF) and Western blot with anti-myc antibody (karyopherin) demonstrates in vivo interaction of SRF with KPNA1 and KPNA2 but not with KPNA3–4 in cytosolic compartment of transfected 293 cells. Equal amounts of total protein were loaded in each input (I) lane. P refers to the pellet that is pulled down by the antibody-coated beads.