Figure 4.

Immunofluorescent double labeling of KRT14 and mucin-5B (MUC5B) or lysozyme in human sinus submucosal glands (SMGs). (A–H) Double labeling of KRT14 and MUC5B. (I–P) Double labeling of KRT14 and lysozyme. Labeling by the KRT14 antibody was detected with a rhodamine-labeled secondary antibody (red). Nulear was stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Labeling of MUC5B and lysozyme were detected with FITC-labeled secondary antibody (green) in the same section. Double labeling revealed no colocalization of KRT14 and MUC5B or lysozyme in SMG cells. Instead, KRT14 was expressed in myoepithelial cells adjacent to the acini. Scale bars, 20 μm in A–L; 10 μm in M–P. The images in the white dotted box in D and L are magnified and shown in E–H and M–P, respectively.