. Author manuscript; available in PMC: 2011 Dec 1.

Published in final edited form as: Cell Metab. 2010 Dec 1;12(6):675–682. doi: 10.1016/j.cmet.2010.11.012

Table 2. Relative mitochondrial mRNA expression and relative mtDNA copy number.

qPCR derived ΔΔCT values for mitochondrial RNAs from age matched Polg^D257A/D257A (n=4, mice P4–P7) and Polg^wt/wt littermates (n=4, mice W3–W6). Two tail t-test indicated all ΔCT values to be significant (p=0.014 to 6.4E-05). Relative mtDNA copy number was determined using Southern blotting. Data is also presented normalized to relative mtDNA copy number “(normalized)”.

	mt-Co1	mt-Nd1	mt-Nd5	mt-Nd6	Control region	mtDNA
Brain	1.91	1.37	1.61	1.67	4.83	0.55
Liver	3.24	1.83	2.56	2.96	1.65	2.29
Brain (normalized)	3.47	2.49	2.93	3.04	8.78	1
Liver (normalized)	1.41	0.80	1.12	1.29	0.72	1