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. 2011 Sep 20;2(5):e00071-11. doi: 10.1128/mBio.00071-11

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Copyright © 2011 Stevens et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 4 — Ribosomal mutations affecting translational accuracy and competence. (A) A strain with an rpsL(K56T) allele (KSP86) displays repressed luciferase activity (triangles) compared to isogenic strains with wild-type (wt) rpsL before the K56T mutation (R895) or after reversion (KSP107). OD₆₂₀s of cultures are shown with squares of matching colors. (B) The rpsL(K56T) mutation decreases suppression of a TAA codon. (C and D) An rpsD(∆K201-L203) ram mutation (strains DCP32 in panel C and DCP31 in panel D) increases luciferase activity (triangles) compared with that of wild-type rpsD (strains DCP19 in panel C and KSP190 in panel D) in the background of wild-type rpsL (C) or rpsL(K56T) (D) alleles. All strains in these panels express the ssbB′-luc competence reporter. (E) Effects of rpsL and rpsD mutations on suppression of a TAA codon. *, P < 0.001 by ANOVA with Bonferroni’s correction.