Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Expression of MtrE by wild-type and mtr120 mutant strains. Western blot analysis of whole-cell lysates from late-log-phase cultures was conducted using polyclonal rabbit MtrE-specific antibodies, followed by goat anti-rabbit IgG-alkaline phosphatase. Strain RD1 (44) contains a Kmr insertion in mtrE and was used as a negative control. The total protein from all strains was equally loaded, as assessed by Coomassie blue staining of a separate SDS-PAGE gel (data not shown).
