TABLE 3 .
The MIC of azithromycin and frequency of recovery of mutations in the promoter region of mtrR and the coding region of mtrR and the mtr120 mutation in 113 N. gonorrhoeae clinical isolates from 2002 to 2009 and 8 WHO N. gonorrhoeae reference strains from 2008
| Azithromycin MIC (µg/ml) (no. of isolates) | No. of isolates with: |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
mtrR promoter mutation |
Nonsynonymous mutation in mtrR coding region |
mtr 120 | |||||||||||
| ΔAa | C→Ab | Tc | Gd | Te | A→Cf | G45D/G45S | A86T | Y105H | D79N | A39T | L99G/L99H | ||
| 0.125 (2) | 1 | ||||||||||||
| 0.25 (4) | 4 | 2 | 4 | ||||||||||
| 0.38 (14) | 7 | 3 | 2 | 9 | 2 | 5 | 2 | 2 | |||||
| 0.5 (9) | 4 | 2 | 1 | 7 | 2 | 2 | 1 | 1 | 1 | ||||
| 0.75 (43) | 34 | 1 | 1 | 2 | 10 | 41 | 12 | 1 | 2 | ||||
| 1 (30) | 26 | 1 | 3 | 29 | 3 | 1 | 2 | ||||||
| 1.5 (8) | 7 | 1 | 8 | 1 | |||||||||
| 2 (4) | 2 | 1 | 1 | 4 | 1 | ||||||||
| 4 (2) | 2 | 2 | |||||||||||
| 6 (4) | 1 | 3 | 3 | 3 | |||||||||
| 8 (1) | 1 | 1 | 1 | ||||||||||
| Total (121) | 86 | 6 | 2 | 1 | 2 | 1 | 24 | 109 | 25 | 9 | 7 | 3 | 1 |
a
Deletion of A in 13-bp inverted repeat in the mtrR promoter.
b
Transversion from C to A 19 nucleotides upstream of where the A deletion occurs.
c
Insertion of one T 10 nucleotides downstream of where the A deletion occurs.
d
Deletion of one G 34 nucleotides upstream of where the A deletion occurs.
e
Deletion of one T 21 nucleotides upstream of where the A deletion occurs.
f
Transversion from A to C in the inverted repeat 3 nucleotides upstream of where the A deletion occurs.