TABLE 4 .
Oligonucleotide primers used in this study
Name | Sequence | Purpose |
---|---|---|
KH9#10B | 5′ CCAAAACCGAAGCCTTGAAAACCAA 3′ |
mtrR::Kmr amplification |
CEL1 | 5′ GACAATGTTCATGCGATGATAGG 3′ |
mtrR::Kmr amplification |
C6 | 5′ CGACATTCCATTCGTCTTCCGG 3′ |
mtrA::Kmr amplification |
C7 | 5′ GCCACGACGGAAAATGCGGAG 3′ |
mtrA::Kmr amplification |
PEmtrC181 | 5′ CCTTAGAAGCATAAAAAGCCAT 3′ | Primer extension of mtrC |
mtrC_3 | 5′ AGTCGGATCCGGTTTGACGAGGGCGGAT 3′ | Full mtrC-lacZ fusion |
mtrC_4 | 5′ AGTCGGATCCAATTGAGACTGCATCT CAACT 3′ | Truncated mtrC-lacZ fusion |
PmtrCmut |
5′ AGTGGATCCGTTTCGGGTCGGTTTGACGAGGG CGGATTATAAAAAAGACTTTTTATCCGTGCAA TCGTGTATGTAGCACGAAACCCA 3′ |
Wild-type mtrC promoter −10 mutation for lacZ fusion |
mtrC_7 | 5′ AGTCGGATCCGAAGCATAAAAAGCC 3′ | Reverse mtrC promoter primer |
mtrC_F | 5′ CGTTTCGGGTCGGTTTGACG 3′ |
mtrR-mtrC intergenic region amplification |
mtrC_R | 5′ CATCGCCTTAGAAGCATAAAAAGCC 3′ |
mtrR-mtrC intergenic region amplification |
MTR1 | 5′ AACAGGCATTCTTATTTCAG 3′ |
mtrR
amplification |
MTR2 | 5′ TTAGAAGAATGCTTTGTGTC 3′ |
mtrR
amplification |