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. 2011 Sep 1;25(17):1807–1819. doi: 10.1101/gad.17325211

Figure 6.

Figure 6.

DC-associated TIN2 mutations interfere with HP1 binding. (A) Two-hybrid analysis of the interaction between DC-associated TIN2 mutations and HP1γ and TRF1. Two-hybrid interactions were scored according to the number of minutes required for the color change: 20 min (++), 45 min (+), and 150–180 min (+/−). (B) Coimmunoprecipitation analysis of the interaction between DC-associated TIN2 mutations and HP1γ. HT1080 stable cell lines expressing HP1γ or vector (V) were transfected with the indicated FlagTIN2 plasmids. Cell lysates were immunoprecipitated with anti-myc beads and analyzed by immunoblotting with anti-myc or anti-TIN2 701 antibody.