Figure 2.

Overexpression of miR-11 suppresses dE2f1-induced apoptosis in transgenic animals. (A) Third instar larval eye discs of various genotypes were incubated with BrdU for 90 min at room temperature, followed by fixing and staining with antibodies recognizing BrdU (red) and active caspase (C3) (green). Analysis was performed on a minimum of 10 larvae of each genotype. The position of the MF is marked with an arrowhead. (B,C) Flies carrying Act88F-Gal4 and UAS-dE2f1 (B) or UAS-ced3 (C) transgenes were crossed to either a wild-type chromosome (Canton S) or UAS-miR-11. (D) RNA was extracted from third instar larval eye discs from GMR-Gal4 crosses described in A, and transcriptional profiles were determined by Affymetrix gene expression microarrays (see the Materials and Methods). Differentially expressed genes were compared for enrichment of GOBP categories with a false discovery rate (FDR) standard cutoff of 0.05 for differentially expressed genes relative to GMR-Gal4/+. Heat map color values represent statistical significance of enrichment (see the scale bar).