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. Author manuscript; available in PMC: 2011 Sep 19.
Published in final edited form as: J Biol Chem. 2005 Jul 26;280(40):34210–34217. doi: 10.1074/jbc.M503523200

FIGURE 2. Validation of IRF6 antibody (a) and IRF6 and maspin (b– d) expression profile in normal primary and cultured mammary epithelial cells.

FIGURE 2

Post-nuclear cytosolic fractions from 1436N1 cells and Ad5 IRF6-infected MDA-MB-231 cells were evaluated by Western blot (a) before and after preincubation of the antibody with blocking peptides, demonstrating the specificity of the IRF6 antibody. Shown are semiquantitative PCR (b), Western blot analysis (c), and confocal microscopy (d) of IRF6 and maspin in normal primary human mammary epithelial cells (HMEpC) and the immortalized mammary cell line 1436N1. Protein from the cytosolic fraction was evaluated by SDS-PAGE on a 10% gel. Actin was used as a protein loading control. GAPDH was used as an RNA loading control. For confocal microscopy, 1436N1 cells were dual-stained for maspin (green) and IRF6 (red; original magnification ×40).