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Photomicrographs showing immunohistochemistry for c-Fos and oxytocin. A: Details of double staining shown at a high magnification. Distribution of c-Fos-positive nuclear profiles and oxytocin cells visualized in coronal sections of the PVN (B) or SON (C). Rats were given either sucrose (B and C top) or cornstarch (B and C bottom) diets for 21 days preceding the perfusion. Open arrows, oxytocin neurons devoid of c-Fos; thin arrows, c-Fos-IR oxytocin neurons; otr, optic tract.
