Figure 1.

hESC-derived retinal cells at 3 weeks (A–C) and 9 weeks (D–L). At 3 weeks, RD cells expressed Sox2 (A, red), Pax6 (B, C, green), and HuC/D (C, red). (D, E) Floating retinal aggregates at 9 weeks with rosettes and RPE. On immunostaining, the centers of the rosettes express Sox2 (F, G, red), Sox9 (F, H, green), and Pax6 (F, I, white; F, merge). The rosettes also express Nrl (J, red) and Recoverin (K, red). (L) HuC/D (green) expressing ganglion and amacrine cells within and around Sox9-expressing neural progenitors (red) in rosettes.