Figure 5.

RT²-PCR of sPLA2-IIa mRNA expression on the ocular surface. CNJ or CN samples from control or DE mice were pooled per group (n = 5) for total RNA isolation and purification. A total of four groups of mice from control or DE mice were assayed. Ten (10) ng of total RNA isolated from CNJ, CN, or LG were reverse transcribed and used for RT²-PCR. Each pooled total RNA sample ran 6 repeats of RT²-PCR with sPLA2-IIa primers, and results were normalized with GAPDH. The results are presented as a mean value ± SD.