Figure 6.

sPLA2-IIa amplified PGE2 productions. Conjunctiva from untreated normal mice were preincubated in 200 μL Shem's medium with 5% FBS at 37°C and 95% air and 5% CO₂ for 1 hour. After the preincubation, medium was changed to either 10 ng/mL TNF-α or 10 ng/mL IL-1β at designated wells for a 1-hour presensitization. Then the CNJ organs were treated for additional 12 hours with fresh Shem's medium containing either human recombinant sPLA2-IIa, TNF-α, and IL-1β alone, or the combinations of sPLA2-IIa with TNF-α or sPLA2-IIa with IL-1β, plus indicated amount of S3319 inhibitor. Each treatment group contained at least two repeats. Each experiment was measured at least three times for PGE2 production. Results of one representative experiment were plotted. All values are the mean ± SD.