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. 2011 Jun 7;52(7):3962–3969. doi: 10.1167/iovs.10-6866

Figure 3.

Figure 3.

Distribution of nmMyH IIB versus α-actinin. Cryostat sections from the midbelly of the developing orbits were stained with antibodies to nmMyH IIB (A, C) and α-actinin (B, D). At P10, the majority of nmMyH IIB was found at the peripheral edge of the fibers. A small percentage of the fibers exhibited an internal distribution (note arrows and insets in A and B). By P15 (C, D), the intrafiber staining for nmMyH IIB became pronounced in the global layers of the EOMs (note arrows and insets). Insets in all panels display higher magnification images of fibers displaying the sarcomeric distribution of nmMyH IIB. Scale bars, 10 μm (A–D); 5 μm (insets).