Figure 2.

SHG in the mouse cornea. 2PM imaging was performed in an unfixed, enucleated C57BL/6 mouse eye imaged with the cornea toward the objective lens. (A) A single coronal cross-section showing the corneal stroma extracellular matrix imaged by SHG. The SHG signal exhibited an interlocking pattern that has been similarly reported in the literature. This 2PM image was captured at a depth of 90 μm from the anterior surface. (B) A virtual transverse cross-section built from a 90-μm deep image stack of 2PM images taken through the cornea. Scale bars, 100 μm.