Figure 7.

Curcumin attenuated SS-induced loss of amacrine cells both in the GCL and INL. After injecting indicated concentrations of SS, curcumin (CC), or vehicle (DMSO) into the vitreous humor of C57BL/6 mice, loss of amacrine cells in radial sections was determined by immunofluorescence staining using antibodies against ChAT (A). Immunofluorescence staining and quantification of cells indicate that, whereas ChAT-positive amacrine cells remained similar in vehicle- or curcumin-treated animals (A, B), ChAT-positive amacrine cells were reduced significantly in animals treated with SS alone (#, ##P < 0.05). In contrast, SS-induced cell loss was reversed (*P < 0.05) when animals were treated with both SS (100 nM) and curcumin (10 μM), but not with SS (12.5 nM) and curcumin (2.5 μM). NS, not significant.