Figure 3.

HT increases tip cells in OIR. OIR mice were treated with hyperoxia (75% oxygen; HT) or were maintained in room air (OIR) from P14 to P17. Retinas were whole flatmounted at P17. Representative images of retinal tip cells (white asterisk) and filopodia (yellow arrow) are shown. (A, B) OIR and HT. Original magnification, ×20. (C, D) High-magnification image from top of A and B. Original magnification, ×40. (E) Quantification of numbers of tip cells (n = 3 retinas from 3 mice). *P < 0.05 compared with OIR.