Blocking VEGF inhibits NV and revascularization in OIR but does not prevent the disruption of the astrocyte network. OIR mice were intravitreally injected with PBS (Veh) or VEGF blocker (VEGFR1/Fc, 2 μg/eye) at P14. Retinal vasculature (isolectin B4, red) and astrocyte network (GFAP, green) were analyzed at P17 in flatmount. (A) Representative images of retinal flatmount and quantification of NV and avascular areas (n = 4 retinas from 4 mice). *P < 0.05 compared with Veh. Original magnification, ×5. (B) Representative confocal images of astrocyte in the central retina are shown (n = 4 retinas from 4 mice). Original magnification, ×20.