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Outline of the present experiments. Eye cups prepared from Sprague-Dawley rats were placed at the bottom of a glass cylinder filled with aCSF at 30°C for 24 hours. The medium was bubbled with 95% O2 and 5% CO2. Hydrostatic pressure at the bottom of the cylinder was calculated to be 10, 35, and 75 mm Hg when aCSF was added to a height of 13.5, 47.2, and 101.2 cm. TFB-TBOA and MSO were added to the aCSF medium during some experiments.
