Figure 10.

(a, b) Real-time RT-PCR analysis of GLAST (a) and GS mRNA (b). (a) GLAST mRNA expression decreased in a pressure-dependent manner and was significantly depressed at 75 mm Hg compared with the control pressure (10 mm Hg). Treatment with 1 and 2 mM MSO induced no remarkable difference in GLAST mRNA expression. (b) GS mRNA was significantly depressed at 75 mm Hg compared with control pressure. Administration of TFB-TBOA (20 and 40 nM MSO) also reduced GLAST mRNA expression at 10 mm Hg. (c) GS activity was significantly depressed at 75 mm Hg compared with control pressure (10 mm Hg). Treatment with 2 mM MSO, 20 nM TFB-TBOA, and 40 nM TFB-TBOA significantly depressed GS activity at 10 mm Hg.