Figure 2.

(a) Light micrograph of the posterior region of the eye cup preparation incubated at 10 mm Hg. The NFL was traced as a layer between the ILM and the GCL. Solid line: the ILM; dashed line: posterior boundary of the NFL. We constructed a perpendicular line (line 1) through the center of the optic disc, and the distance was measured along the ILM from the crossing point (point A; white circle) of the ILM and line 1. It is easy to identify the NFL by light microscopy within the retinal region ∼1000 μm proximal to point A. (b) High magnification of the rectangular area depicted in (a). Line a traces the ILM and the posterior border of the retinal vessels. The prominent NFL can be easily traced in this region, but is gradually diminished in thickness, especially in the retinal region 1200 μm distal from point A. In the present study, we examined the NFL thickness of the retinal region greater than 1200 μm away from point A. (c, d) The NFL of retinas incubated at 10 and 75 mm Hg. The lines are as described in (a). The images in (c) and (d) correspond to the micrographs of in Figures 4d and 4c, respectively. (d) Measurement of the NFLT in the retinal region greater than 1200 μm away from point A. The five lines perpendicular to the pigment epithelial layer were constructed at a distance of 15 μm from each other, and the crossing points with the ILM (line a), the posterior boundary of the ILM (line b), and the pigment epithelial layer (line c) are marked as points c, d, and e, respectively. The distance between points c and d is defined as the NFLT (α), and the distance between points c and e as total RT (β). The percentage of NFLT is measured as α/β × 100 (%). Scale bar: (a) 40 μm; (b, c) 12 μm; (d) 15 μm.