Figure 8.

Immunofluorescent localization of GLAST (a, c, d–h), vimentin (b), and GS (i–m) by laser scanning microscopy. Cryosections are counterstained with DAPI in (c). (a–c) Sequential localization (a, b) and colocalization (c) of GLAST and vimentin. (a) GLAST (green; FITC) was expressed throughout the retina. Arrowheads: GLAST-positive radial staining throughout the retina. (b) Arrowheads: vimentin-positive Müller cell and radial staining (red; rhodamine). (c) The GLAST signals (green; FITC) coincide with the vimentin staining (red; rhodamine). Arrows: the colocalization of GLAST and vimentin in radial staining. (d–f) GLAST expression (green; FITC) decreased after incubations at 10 (d), 35 (e) and 75 (f) mm Hg in a pressure-dependent manner. (g, h) Administration of 1 (g) and 2 (h) mM MSO showed similar GLAST immunofluorescence intensity compared with controls incubated at 10 mm Hg. (i–m) GS (green; FITC) expression by the Müller cell end foot and cell body decreased in a pressure-dependent manner (i,10; j, 35; and k, 75 mm Hg). (l, m) Administration of 20 (l) and 40 (m) nM TFB-TBOA markedly decreased fluorescence compared with controls incubated at 10 mm Hg. Scale bar, 20 μm.