Figure 2.

Generation of transgenic rats and mice with targeted overexpression of SOCS1 in the retina. (A) Derivation of a 1500-bp fragment consisting of an opsin promoter element fused to murine SOCS1 cDNA and an enhancerless–promoterless SV40 polyadenylation sequence. The chimeric cDNA fragment was microinjected into the pronuclei of one-cell mouse or rat embryos. Western blot analysis of whole cell extracts isolated from lens or retina of WT or SOCS1 transgenic (SOCS1-Tg) rat (B) or retina of WT and SOCS1-Tg mice (C). (D) Retinal cells from WT or SOCS1-Tg mice were stimulated with IFN-γ and pSTAT1 expression was detected by Western blot analysis.