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K. pneumoniae capsule. Transmission electron microscopy visualization of capsule production from NTUHK2044 (A), ΔmagA (B), and magA complement (C). Sedimentation of overnight cultures at 10,000g for 10 minutes (D). Semiquantitative RT-PCR detecting magA and 16S rRNA (E). Lanes 1–3, reverse transcriptase; lanes 4–6, no reverse transcriptase; lane M, 100-bp DNA ladder; lanes 1 and 4, NTUHK2044; lanes 2 and 5, ΔmagA; lanes 3 and 6, magA complement; lane 7, NTUHK2044 genomic DNA control.
