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. 2011 Aug 17;52(9):6545–6551. doi: 10.1167/iovs.10-6772

Table 2.

Effect of Cytokines on Pro- and Antisurvival Gene Expression in mRPE Cells

Gene mTNF-α hIL-1β hTNF-α
Prosurvival
    Survivin 0.9 ± 0.6 1.1 ± 0.8 0.6 ± 0.3*
    Bcl-xL 2.3 ± 0.9* 1.9 ± 0.7 1.9 ± 0.6*
    c-FLIP 2.3 ± 0.5** 2.0 ± 0.3** 3.3 ± 2.0
    TRAF-2 3.5 ± 0.8** 3.8 ± 1.0** 2.9 ± 0.3
    c-IAP1 6.2 ± 0.9** 3.9 ± 0.6* 7.3 ± 4.1
    c-IAP2 12.2 ± 2.5** 6.8 ± 1.9* 17.8 ± 3.3**
    TRAF-1 57.0 ± 42.9* 15.1 ± 17.1 120.2 ± 47.5*
Antisurvival
    Bad 1.9 ± 0.4* 2.2 ± 1.0 2.2 ± 1.6
    Bax 1.5 ± 0.2** 1.5 ± 0.2** 1.2 ± 0.6**
    Bik 1.6 ± 0.6** 1.2 ± 0.2** 2.7 ± 1.8
    Bak-1 3.7 ± 1.0* 2.7 ± 1.2* 4.7 ± 2.0*
    Bid 9.1 ± 1.2** 3.1 ± 0.9** 13.4 ± 4.0**

Pooled mRPE cells were treated with either medium alone (basal), m- or hTNF-α (22 ng/mL), or hIL-1β (5 U/mL) in serum-free DMEM for 24 hours. RNA was extracted and reverse transcribed to synthesize cDNA. The transcripts were amplified using gene-specific primer pairs and quantified using qRT-PCR. Numbers correspond to the fold-difference between cytokine-treated gene expression and basal gene expression, after normalization to the reference gene, PPIA.

Data are the mean ± SD of three independent experiments.

*

P < 0.05 vs. untreated cells;

**

P < 0.005 vs. untreated cells.