Table 2.

Effect of Cytokines on Pro- and Antisurvival Gene Expression in mRPE Cells

Gene	mTNF-α	hIL-1β	hTNF-α
Prosurvival
Survivin	0.9 ± 0.6	1.1 ± 0.8	0.6 ± 0.3*
Bcl-xL	2.3 ± 0.9*	1.9 ± 0.7	1.9 ± 0.6*
c-FLIP	2.3 ± 0.5**	2.0 ± 0.3**	3.3 ± 2.0
TRAF-2	3.5 ± 0.8**	3.8 ± 1.0**	2.9 ± 0.3
c-IAP1	6.2 ± 0.9**	3.9 ± 0.6*	7.3 ± 4.1
c-IAP2	12.2 ± 2.5**	6.8 ± 1.9*	17.8 ± 3.3**
TRAF-1	57.0 ± 42.9*	15.1 ± 17.1	120.2 ± 47.5*
Antisurvival
Bad	1.9 ± 0.4*	2.2 ± 1.0	2.2 ± 1.6
Bax	1.5 ± 0.2**	1.5 ± 0.2**	1.2 ± 0.6**
Bik	1.6 ± 0.6**	1.2 ± 0.2**	2.7 ± 1.8
Bak-1	3.7 ± 1.0*	2.7 ± 1.2*	4.7 ± 2.0*
Bid	9.1 ± 1.2**	3.1 ± 0.9**	13.4 ± 4.0**

Pooled mRPE cells were treated with either medium alone (basal), m- or hTNF-α (22 ng/mL), or hIL-1β (5 U/mL) in serum-free DMEM for 24 hours. RNA was extracted and reverse transcribed to synthesize cDNA. The transcripts were amplified using gene-specific primer pairs and quantified using qRT-PCR. Numbers correspond to the fold-difference between cytokine-treated gene expression and basal gene expression, after normalization to the reference gene, PPIA.

Data are the mean ± SD of three independent experiments.

^*P < 0.05 vs. untreated cells;

^**P < 0.005 vs. untreated cells.