Figure 4.

Failure of lens detachment in Spry mutants. Sections of E10.5 (A–D), E11.5 (E–H′), E12.5 (I–L′), and E15.5 (M–P′), control (Cre−, A, E, E′, I, I′, M, M′), Spry2^fl/fl; Cre (B, F, F′, J, J′, N, N′), Spry1^fl/+;Spry2 ^fl/fl;Cre (C, G, G′, K, K′, O, O′), and Spry1^fl/fl;Spry2^fl/fl;Cre (D, H, H′, L, L′, P, P′) embryos were analyzed by hematoxylin and eosin staining. (E′–P′) Higher magnifications of (E–P). Lens placode invagination (B–D) was unaffected in Spry1 and -2 mutants. At E11.5, Spry mutants showed prominent stalks (F–H′) in contrast to control embryos (E′). At E12.5 and at E15.5, Spry mutant lenses failed to separate and displayed persistent lens stalks (J–L′, N–P′, arrows) in contrast to controls (I, I′, M, M′). Corneal stroma (cs) and endothelium (cen) were discontinuous in Spry mutants at E15.5 (N–P′). ce, corneal epithelium; cs, corneal stroma; cen, corneal endothelium; le, lens epithelium; lf, lens fibers; lp, lens pit; oc, optic cup; r, retina. Scale bar in (M): (A–H) 30 μm; (I–L,M–P) 60 μm; (E′–H′, I′–L′) 10 μm; (M′–P′) 15 μm.