Figure 8.

Lens fiber differentiation in Spry mutant embryos. In situ hybridizations (A, B, E, F) and immunohistochemistry (C–D′, G–H′) were performed on E15.5 control (Cre−) and Spry mutant embryos to detect expression of Prox1 (A–D′) and p57^KIP2 (E–H′). In situ hybridizations were performed using ³⁵S-labeled riboprobes (A, B, E, F). Prox1 (B, D, D′, arrows), and p57^KIP2 (F, H, H′, arrows) were upregulated in Spry mutant lens epithelial cells. Some of the stalk cells also expressed p57^KIP2 (H′, yellow arrow). ce, corneal epithelium; le, lens epithelium; lf, lens fibers; r, retina. Scale bar in (G′): (A–D, E–H) 60 μm; (C′, D′, G′, H′) 10 μm.