Mislocalization of opsins and upregulation of GFAP in mRDef transgenic mice. (A) Immunohistochemical analysis of opsin localization in retinal cryosections with a green cone opsin–specific antibody. (Left) Wild-type, (middle) mRDefRpgr−/− transgenic, and (right) mROrfRpgr−/− transgenic retina. (B) Upregulation of GFAP immunoreactivity in mRDefRpgr−/− retina. (Left) Wild-type, (middle) mRDefRpgr−/− transgenic, and (right) mROrfRpgr−/− transgenic retina. RPE, retinal pigment epithelium; ONL, outer nuclear layer; INL, inner nuclear layer; GC, ganglion cell layer.