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Expression of Gal on pCECs was significantly lower than on pAECs. For flow cytometry, cultured corneal cells (epithelium, stroma, and endothelium), and AECs from (A) WT and (B) GTKO pigs were stained with BSI-B4 to detect Gal. Dotted traces: unstained control. Results are representative of three independent experiments. (C) The expression of Gal on quiescent WT pCECs and pAECs was compared with that on activated cells (n = 6; *P < 0.05).
