Figure 2.

Minimum expression of Gal in WT pig corneas. Fluorescence staining for Gal (BSI-B4-FITC: green) and nuclei (DAPI: blue) in corneas from (A) WT pigs, (B) GTKO pigs, and (C) humans was determined by fluorescence microscopy (original magnification, ×200). In WT pigs, Gal expression was mainly seen in the limbal area and gradually diminished toward the central cornea. Weakly Gal-positive cells were recognized in keratocytes (red arrows) in the anterior stroma. In contrast to WT pig corneas, there was no expression of Gal in GTKO pig and human corneas. Results are representative of three independent experiments.