Figure 15.

Contribution of the Bruch's membrane explant (choroid and sclera) to protein secretion (no fRPE or hES-RPE seeded). Contribution of protein secretion into the media from equatorial (N = 4) or submacular (N = 5) explants was determined after incubation for 21 days in stem cell media or RPE media. Secretion levels were corrected for levels in media only and are expressed as mean log pg/mL (error bars = SEM). Secretion was significantly different between pairs noted with an asterisk (P < 0.05) for PEDF and TGFβ2.