Figure 2.

Qdot labeling of human anterior segments. (A) Human anterior segment viewed en face from the perspective of the anterior chamber showing the distribution of Qdots (red) in approximately one quadrant of a human eye. Presumably, the intensity of Qdot labeling is a direct indicator of outflow, such that regions of dense, moderate, and sparse labeling correspond to regions of high, medium, and low outflow through the TM. (B) Radial section through a high-labeled region of a human eye showing the distribution of Qdots in the TM (red). DAPI was used to stain nuclei (blue). SC, Schlemm's canal. (C) Comparison of Qdot labeling (green) and cell membrane stain (red) viewed en face in a high (C) and low (D) Qdot-labeled area. Scale bar; (B) 20 μm; (C, D) 60 μm.