Figure 7.

OTC protects ARPE-19 cells against H₂O₂-induced cell death. (A) Oxidative stress was induced in ARPE-19 cells using H₂O₂ (500 μM) in the presence or absence of OTC (0.25, 0.5, and 1 mM). Cell viability was analyzed by trypan blue exclusion assay (*P < 0.01 compared to control; **P < 0.01 compared to H₂O₂-treated cells). (B, C) Oxidative stress was induced in ARPE-19 cells using H₂O₂ (500 μM) in the presence or absence of OTC (1 mM). Cells were then used for Annexin V-FITC apoptosis assay (*P < 0.001 compared to control; **P < 0.001 compared to H₂O₂-treated cells) and glutathione measurements (*P < 0.001 compared to control; **P < 0.001 compared to H₂O₂-treated cells).