Table 1.
Sequence of PCR Primers
| Gene | Primer Sequences | Annealing Temperature (°C) | Expected Product Size (bp) |
|---|---|---|---|
| γ-GTPase | FWD: 5′-GATTCGGCACCACCATACAG-3′ | 56 | 160 |
| REV: 5′-TGGCTGCGGGTCAATCT-3′ | |||
| GS | FWD: 5′-CTAATGCGGTGGTGCTACTG-3′ | 55.2 | 278 |
| REV: 5′-ACACTTGGCAGCACGAGA-3′ | |||
| GCLM | FWD: 5′-GGCATGCTCCGTCCTT-3′ | 54.4 | 327 |
| REV: 5′-AGCAGTTCTTTGGGTCATT-3′ | |||
| GCLC | FWD: 5′-ATGGGGCTGCTGTCCCAAGG-3′ | 56 | 219 |
| REV: 5′-CCTTGGGACAGCAGCCCCAT-3 |
GCLM, γ-glutamatecysteine ligase modulatory subunit; GCLC, γ-glutamatecysteine ligase catalytic subunit; GS, glutathione synthetase; γ-GTPase; γ-glutamyltranspeptidase.