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Concentration response of spiradoline (SPR) on NO production in NPCE (A) or HTM-3 (B) cells. Cells were treated with the indicated concentrations of SPR or estradiol (ES) for 24 hours. At the end of drug treatments, NO in the incubation medium was analyzed as [nitrates + nitrates] using a microplate assay kit. Data are mean ± SEM of 4 to 10 experiments in triplicate. *P < 0.05 compared to control.
