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Effect of norBNI on SPR-stimulated NO production in NPCE (A) or HTM-3 (B) cells. Cells were pretreated with norBNI for 30 minutes, followed by treatment with SPR for 24 hours. At the end of drug treatments, NO in the incubation medium was analyzed as [nitrates + nitrites] using a microplate assay kit. Data are mean ± SEM of four to nine experiments in triplicate. *P < 0.05 compared to control. #P < 0.05 compared to SPR alone.
