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Effect of L-NAME on SPR-stimulated NO production in NPCE (A) or HTM-3 (B) cells. Cells were pretreated with L-NAME for 30 minutes, followed by treatment with SPR for 24 hours. At the end of drug treatments, NO in the incubation medium was analyzed as [nitrates + nitrites] using a microplate assay kit. Data are mean ± SEM of 4 to 11 experiments in triplicate. *P < 0.05 compared to control. #P < 0.05 compared to SPR alone.
