Table 1 . I-Sce1 resistant end joining junctions.
| Microhomology (nt) | WT: no. of clones | Trex2null: no. of clones | |
|---|---|---|---|
| I-SceI site (CAPS) | |||
| attcTAGGGATAACAGGGTAATggat | |||
| Insertions/deletions | |||
| Insertion + 1 | 1/23 | 1/23 | |
| TAGGGAATAACAGGGTAAT + 1 insertion | 0 | 1 | |
| TAGGGataacATAACAGGGTAAT + 5 insertions | NA | 1 | |
| Deletion, 1–5 nt | 2/23 | 2/23 | |
| TAGGGAT…ACAGGGTAAT, 1-nt deletion | 0 | 1 | |
| TAGGG…TAACAGGGTAAT, 1-nt deletion | 0 | 1 | |
| TAGGGA…ACAGGGTAAT, 2-nt deletion | 1 | 1 | |
| TAGGG…AACAGGGTAAT, 2-nt deletion | 1 | 1 | |
| Deletion, 6–10 nt | 14/23 | 12/23 | |
| ttc…TAACAGGGTAAT, 6-nt deletion | 2 | 1 | |
| TAGGGA…TAATGGAT, 8-nt deletion | 3 | 3 | |
| TAGGG...TAAT, 9-nt deletion | 4 | 9 | 9 |
| agaa…ATAACAGGGTAAT, 9-nt deletion | 2 | 1 | |
| TAGGGAT…Atgg, 9-nt deletion | 1 | 1 | |
| aatt…ACAGGGTAAT, 9-nt deletion | 0 | 1 | |
| ttc...AGGGTAT, 10-nt deletion | 1 | 1 | |
| Deletion, >10 nt | 6/23 | 8/23 | |
| agaa…CAGGGTAAT, 12-nt deletion | 2 | 1 | |
| ttcTA…ggatcc, 16-nt deletion | 2 | 1 | |
| agaa…TAAT, 17-nt deletion | 1 | 1 | |
| ttgg…CAGGGTAAT, 19-nt deletion | 2 | 1 | |
| TAGGG…tcatggtcg, 22-nt deletion, 4-nt insertion | 0 | 1 | |
| catc…AACAGGGTAAT, 24-nt deletion | 1 | 1 | |
| TAGGG…cgcc, 25-nt deletion | 0 | 1 | |
| atca…GGGTAATGG, 27-nt deletion | 2 | 1 | |
| attt…Tggat, 30-nt deletion | 2 | 1 | |
| TAGGGA…atgg, 31-nt deletion | 0 | 1 | |
| tcat…cacc, 41-nt deletion | 2 | 1 | |
| tgct…GGGTAAT, 48-nt deletion | 2 | 1 | |
| tttt…CAGGGTAAT, 70-nt deletion | 1 | 1 | |
| gcaa…AGGGTAAT, 79-nt deletion | 2 | 1 | |
Boldface type, inserted sequence; underlined, microhomology; NA, not applicable.