Figure 3 .

The pkc1 mutants and cells lacking downstream components of Pkc1 are mildly sensitive to H₂O₂. (A) Fivefold serial dilutions (from left to right starting from OD₆₀₀ = 1) of pkc1Δ mutant carrying YCp–PKC1 (DL106), YCp–pkc1-1 (DL511), YCp–pkc1-2 (DL506), and YCp–pkc1-3 (DL504) were grown at 25, 30, and 33° for 2–4 days on SC−Ura plates containing 1 M sorbitol after treatment with 2 mM H₂O₂ for 200 min or mock treatment. The results were about the same at all temperatures tested, and only the plate at 30° is shown. (B) Cells of bck1Δ (DL253) and mpk1Δ (JVG216) mutants were treated similarly, except plated on YPD plates at 30°.