Figure 9 .

rho1^ts mutants are hypersensitive to cadmium while ycf1Δ mutant are slightly sensitive to H₂O₂. (A) Fivefold serial dilutions (from left to right, starting from OD₆₀₀ = 2) of wild-type (NY2284), ycf1Δ (HPY1738), rho1-5 (NY2287), rho1-2 (NY2285), and rho1-3 (NY2286) strains, all of which are in the isogenic background, were grown on SC or SC plate containing 30 μM CdCl₂ at room temperature for 4 days (−) or 7 days (+30 μM CdCl₂). (B) Fivefold serial dilutions (from left to right, starting from OD₆₀₀ = 1) of wild type (BY4741) and isogenic deletion mutants of vacuolar transporters (ycf1Δ, ybt1Δ, and bpt1Δ) were treated with 2 mM H₂O₂ or mock treated, spotted on SC plates, and incubated at 30° for 2 days. (C) Fivefold serial dilutions (from left to right, starting from OD₆₀₀ = 1) of wild type (NY2284), ycf1Δ (HPY1738), rho1-5 (NY2287), and rho1-5 ycf1Δ (HPY1739) were treated as in Figure 9B.