Table 1. Yeast strains used in this study.
| Strain | Relevant genotypea | Source/comments | |
|---|---|---|---|
| NY2284* | α | ura3 leu2 trp1 his3 ade2 lys2 rho1Δ::HIS3 ade3::RHO1::LEU2 | Guo et al. (2001) |
| NY2285* | a | rho1Δ::HIS3 ade3::rho1-2E45V::LEU2 | Guo et al. (2001) |
| NY2286* | α | rho1Δ::HIS3 ade3::rho1-3L60P::LEU2 | Guo et al. (2001) |
| NY2287* | a | rho1Δ::HIS3 ade3::rho1-5G121C::LEU2 | Guo et al. (2001) |
| HPY1710* | α | YCF1-VN::kanMX6 | See text |
| HPY1737* | α | tus1Δ::TRP1 YCF1-VN::kanMX6 | See text |
| HPY1738* | α | ycf1Δ::kanMX4 | See text |
| HPY1739* | a | rho1Δ::HIS3 ade3::rho1-5G121C::LEU2 ycf1Δ::kanMX4 | See text |
| HPY1574* | α | RHO1::GFP-RHO1-URA3 | Derived from NY2284b |
| HPY1730* | a | rho1-2::GFP-RHO1-URA3 | Derived from NY2285b |
| HPY1731* | α | rho1-3::GFP-RHO1-URA3 | Derived from NY2286b |
| HPY1732* | a | rho1-5::GFP-RHO1-URA3 | Derived from NY2287b |
| HPY1955* | α | YCF1-GFP::TRP1 | See text |
| EG123# | α | ura3-52 leu2-3,112 trp1-1 his4 can1 | Same as HPY11, Park et al. (1993) |
| DL106# | α | pkc1∆::LEU2 YCp50-PKC1 | Levin and Bartlett-Heubusch (1992) |
| DL511# | α | pkc1∆::LEU2 YCp50-pkc1-1 | Levin and Bartlett-Heubusch (1992) |
| DL506# | α | pkc1∆::LEU2 YCp50-pkc1-2 | Levin and Bartlett-Heubusch (1992) |
| DL504# | α | pkc1∆::LEU2 YCp50-pkc1-3 | Levin and Bartlett-Heubusch (1992) |
| DL253# | α | bck1Δ::URA3 | Lee and Levin (1992) |
| JVG216# | a | mpk1Δ::TRP1 | Krause and Gray (2002) |
| BY4741@ | a | his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Open Biosystems |
| HPY1904@ | a | ycf1Δ::kanMX4 | Open Biosystems |
| HPY1905@ | a | ybt1Δ::kanMX4 | Open Biosystems |
| HPY1906@ | a | bpt1Δ::kanMX4 | Open Biosystems |
| THY AP4^ | a | leu2, ura3, trp1::(lexAop)-lacZ (lexAop)-HIS3 (lexAop)-ADE2 | Obrdlik et al. (2004) |
| YCF1–CT^ | a | YCF1-Cub-LexA-VP16 KanMX | Derived from THY AP4 |
| ArBT–CT^ | a | SHO1::MFαSS-CD4tm-Cub-LexA-VP16 KanMX | Derived from THY AP4 |
| YCF1–CT ΔT^ | a | YCF1-Cub-LexA-VP16 KanMX tus1Δ::NatR | Derived from THY AP4 |
| ArBT–CT ΔT^ | a | SHO1::MFαSS-CD4tm-Cub-LexA-VP16 KanMX tus1Δ::NatR | Derived from THY AP4 |
a
Strains marked with * are isogenic to NY2284, except as indicated; strains marked with @ are isogenic to BY4741, except as indicated; strains marked with # are isogenic to EG123 (Park et al. 1993), except as indicated; strains marked with ^ are isogenic to THY AP4 except as indicated; and the background of the strains marked with * and @ is S288C.
b
pRS306–GFP–RHO1 (pHP1699) was integrated into the RHO1 locus after digestion with BglII.