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. 2011 Aug;188(4):1019–1022. doi: 10.1534/genetics.111.131078

Figure 1.

Figure 1

Silencing of the 22- and 19-kDa α-zeins linked with the GFP marker. (A) pTF102-P6z1RNAi construct structure. The Zp22/6 gene was used for the 22-kDa and z1A2 gene for the 19-kDa inverted repeat (Song and Messing 2002, 2003). The binary vector pTF102 was used for all intermediate construct building and Agrobacterium-mediated transformation (Frame et al. 2002). (B) SDS-PAGE analysis of zein accumulation in progeny from (B73 × Mo17) × P6z1RNAi/+. Six normal and “green” kernels at 18 days after pollination (DAP) were selected with a fluorescence dissection microscope (Leica MZ16 F) for zein extraction. Total zein loaded in each lane was equal to 500 mg of fresh endosperm at 18 DAP. Protein markers (M) from top to bottom are 37, 25, 20, 15, and 10 kDa. (C) Segregation of vitreous and opaque kernels scored with a light box.