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. 2011 Sep;189(1):11–21. doi: 10.1534/genetics.111.130781

Figure 2 .

Figure 2 

Kinetochore mutants are sensitized to nicotinamide (NAM). Strains were serially diluted fivefold and spotted onto YPD plates with 0 mM or 30 mM NAM, incubated at the indicated temperatures, and photographed after 3 days. (A) Wild-type CSE4 (RC154) and temperature-sensitive C-terminal cse4 mutants, cse4-1 (RC147) and cse4-111 (MSY1520). (B) Wild-type HHF1 (MSY559) and temperature-sensitive chromosome segregation histone H4 mutant hhf1-20 (MSY554) (top), and strains expressing wild-type histone H4 (YMB8155), nonacetylatable H4K16R (YMB8156), and acetylmimic H4K16Q (YMB8157) (middle and bottom). (C) Temperature-sensitive kinetochore mutants, ndc10-1 (JK421), mif2-2 (6849-10-1), and nonessential kinetochore mutants, mcm21∆ (YPH1715) and ctf19∆ (YPH1713). Note the ndc10-1 mutant is inviable above 30°.